FITX details

Performance of Automatic Pipetting Module(APM)

1/8 channel- Volume 50 µl
  1 µl 50 µl
Accuracy (Rel.) ± 7% ± 1%
Precision (Rel. CV) ≤ 7.5% ≤ 0.4%
1/8 channel – Volume 200 µl
  10 µl 200 µl
Accuracy (Rel.) ± 3% ± 0.8%
Precision (Rel. CV) ≤ 1% ≤ 0.15%

Note :
According to ISO-8655 standards ( Gravimetic method ), APM is calibrated in temperature (21 ~ 25ºC, ±0.5ºC) and humidity (60~90%) controlled environment. Twice-distilled water, robotic tips and microbalance were used.

 

Excellent Serial Dilution Results.

EzMate™ 401

7μl NIH3T3 Cell cDNA sample is diluted in 21μl water at 1:4 ratio for 4 times. Roche® LightCycler® 480 real-time PCR thermal cycler and Finnzyme DyNAmo® Flash SYBR® Green qPCR kit (F-415L) were used.

Better Precision Results than Manual Pipetting.

Hand pipetting

Target: Human GAPDH ( top curve) was amplified in replicates of 4. 18μl (master-mix) and 2μl (cDNA ) were pipetting into 20μl reaction volume. Roche® LightCycler® 480 real-time PCR thermal cycler and Invitrogen™ Platinum® Taq DNA Polymerase were used.

 

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