Products for Western Blotting

1. Sample Prep

•  Antibody Production – custom polyclonal antibodies or monoclonal antibodies from PrimmBiotech
• Lysis or Tissue Homogenization – homogenize using the Bullet Blender
• Protein quantification - quantitate your protein using the Spectrostar NANO

2. Electrophoresis 

• Acylamide Gels – Pour your own and save money:  Protogel
• Pre-Cast Acrylamide Gels: NuSep precast minigels
• Proteomic buffers : Tris-Glycine-SDS PAGE Buffer (10X), etc from National Diagnostics
• Protein labels and dyes:   Protoblue safe from National Dianostics to replace coomassie stains.

3.  Western Blot Processing  & Detection

• Automate your western blots processing:  BlotCycler™
   automates the process of Western blotting for up to 6 blots at once.   All steps after protein transfer to a membrane are fully automated: shaking, blocking, hybridization, washing, and recycling of the primary antibody.
• Chemiluminescence - the most popular labeling system used for Western blotting.    Detect your chemiluminescence with the Syngene imaging systems or our BMG luminometers.

4. Imaging & Quantitation

• Gel and Blot Imaging:   Syngene imagers
• Image Analysis Software:  densitometry, molecular weight sizing, colony counting using GeneTools analysis software
• Quantification using spectrophotometer / fluorometer