Western Blotting

Products for Western Blotting

1. Sample Prep

2. Electrophoresis 

  • Acylamide Gels – Pour your own and save money:  Protogel
  • Pre-Cast Acrylamide Gels: NuSep precast minigels
  • Proteomic buffers : Tris-Glycine-SDS PAGE Buffer (10X), etc from National Diagnostics
  • Protein labels and dyes:   Protoblue safe from National Dianostics to replace coomassie stains.

3.  Western Blot Processing  & Detection

  • Automate your western blots processing:  BlotCycler™
     automates the process of Western blotting for up to 6 blots at once.   All steps after protein transfer to a membrane are fully automated: shaking, blocking, hybridization, washing, and recycling of the primary antibody.
  • Chemiluminescence – the most popular labeling system used for Western blotting.    Detect your chemiluminescence with the Syngene imaging systems or our BMG luminometers.

4. Imaging & Quantitation

  • Gel and Blot Imaging:   Syngene imagers
  • Image Analysis Software:  densitometry, molecular weight sizing, colony counting using GeneTools analysis software
  • Quantification using spectrophotometer / fluorometer