Worthington Biochemical


Worthington is a privately owned, ISO9001 Certified primary producer of purified enzymes, proteins and nucleic acids for biotech and life science research, regenerative medicine, diagnostic, biopharmaceutical and bioprocessing applications under GMP guidelines.

Vaccine Production & Bioprocessing

Do you need to eliminate BSE/TSE & mammalian viral risks? Worthington offers Animal Free (AF) nucleases & proteases for vaccine and bioprocessing applications.

Products include:
  • Two grades of non-mammalian Animal Free (AF) Recombinant DNase I (pdf)
  • Aspergillus AF RNase T1 & T2
  • Plant & fungal sourced AOF proteases
  • NEW Animal Free Collagenase (CLSAFA, AFB, AFC)
  • NEW STEMxyme® 1 & 2, Animal Free Collagenase/Neutral Protease Blends
  • AF Neutral Protease (Dispase®)
  • RNase-free DNase
  • DNase-free RNase
  • Proteinase K

For classical cell isolation, immunoconjugation, and vaccine production applications:

  • Alkaline phosphatase
  • Trypsins
  • Elastase
  • Neutral Protease

Trypsin was one of the first products commercially produced by Worthington in the late 1940’s, based upon the early enzyme crystallization work of Northrop and Kunitz published in 1931.

Since then Worthington has added the production many other proteases that are used for many diverse life science applications including primary cell isolation and tissue dissociation, cell culture, bioprocessing, diagnostics, regenerative medicine and proteomics.

Worthington produces a chemically modified SequENZ® Trypsin with significantly reduced autolysis when used under extensive protein digests, along with specialty packaged trypsin, chymotrypsin, Endo-Arg-C and Endo-GLU-C for proteomics and protein sequencing applications.

Cell Biology

Working with primary cells? The newly updated Worthington Tissue Dissociation Guide provides a general introduction to the theory and techniques involved in primary cell isolation as well as a set of annotated references organized by tissue type. Worthington also offers the following enzymes and use-tested kits:

  • NEW STEMxyme® 1 & 2, Animal Free Collagenase/Neutral Protease Blends
  • NEW Animal Free Collagenase (CLSAFA, AFB, AFC)
  • AF Neutral Protease (Dispase®)
  • AF DNase I, Recombinant
  • AF RNase T1 & RT2
  • Hepatocyte Isolation System (HIS)
  • Neonatal Cardiomyocyte Isolation System (NCIS)
  • Papain Dissociation System (PDS)
  • Cell Isolation Optimizing System (CIT)
Molecular Biology

Worthington Biochemical has purposely limited our products for molecular biology to the major ‘work-horse’ enzymes and nucleic acids. There are several other companies that specialize in the many restriction enzymes, plasmids, vectors and various kits that are used in molecular biology applications.

In addition, Worthington has developed the technology to consistently produce and purify recombinant enzymes and proteins in genetically engineered bacteria and yeast. Many of these recombinant products are also produced to be free of animal components (Animal Free, AF) to eliminate BSE/TSE & mammalian viral risks. We look forward to continuing development of this technology and welcome your comments and suggestions of how we may better serve your needs.

  • NEW Animal Free (AF) Recombinant DNase I, Molecular Biology Grades (DR1S/DR1) and Bioprocessing Grade (DR2)
  • Animal Free (AF) RNase T1(RTIS) & Recombinant RNase T2 (RT2R)
  • Recombinant HIV Reverse Transcriptase (RTHIV)
  • Phosphodiesterases I/II (VPH/SPH)
  • Mirococcal & S1 Nucleases (NFCP/SINUC)
  • Phosphatases (CAP/BAPF/BAPC)
  • Nucleic Acids (DNA/SDNA/DNAEC/DNAL & fragments)

Worthington is a primary producer of enzymes, biochemicals and kits for life science and neuroscience applications.

While many researchers have successfully used collagenase or trypsin for tissue dissociation, we generally recommend papain for neural cell isolation. The Worthington Papain Dissociation System is a set of reagents intended for use in the tissue dissociation method of Huettner and Baughman (1986). Each lot is use tested for performance in rat spinal neural cell isolation, and this kit provides freshly prepared enzyme solutions for each dissociation:

Plant Biology

Worthington is a primary producer of enzymes, biochemicals and kits. We offer products such as cellulase and pectinase that are useful for a variety of plant biology and plant pathology applications.